Linus Usman Akacha¹, Muluh Emmanuel Khan², John O. Igoli³, JohnVershima Anyam³ and DikkoYuwel Jatau¹

 ¹Department of Chemistry, Adamawa State College of Education, Hong

²Department of Chemistry, Federal University Lokoja

³Department of Chemistry, University of Agriculture, P.M.B 2373 Makurdi, Benue State, Nigeria

Received: Nov 18, 2023/ Revised: Jan 1, 2024/Accepted: Jan 5, 2024

Abstract

 This study aims at the isolation and characterization of bioactive constituents from the stem of Bryophyllum pinnatum in n-hexane extract. Spectroscopic and chromatographic methods were used. The isolation was conducted through vacuum Liquid Chromatography (VLC) in the polarity gradient of solvent systems, Thin Layer Chromatography (TLC) was used to confirm their purity, and purification was carried out using gel filtration chromatography (GFC). Characterization of the isolated compounds was carried out using Nuclear Magnetic Resonance Spectroscopy. Experimental data of active compounds was in agreement with NMR data of β-Sitosterol and ferulic acid available in the literature, Elemental analysis showed that percentages of C, H and O in the isolated compounds conformed with calculated values of those in β-Sitosterol and ferulic acid. Isolation and characterization of β-Sitosterol and ferulic acid was reported for the first time, from the stem of Bryophyllum pinnatum n-hexane extract.

 Keywords: Bryophyllum pinnatum, Microwave, NMR, VLC, GFC, TLC plate.

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How to cite this article

Akacha, L. U., Khan, M. E., Igoli, J. O., Anyam, J. V. and Jatau, D. Y. (2024). β-Sitosterol and ferulic acid from the hexane extract of Bryophyllum pinnatum stems. Chemical and Environmental Science Archives, Vol. 4(1), 1-6.

https://doi.org/10.47587/CESA.2024.4101

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